How It Works

DNA-based Technology

  • DNA barcode attached to primary antibodies
  • Complementary labels attaches to fluorophores
  • Specific, sensitive and reversible

Streamlined workflow

  • Same sample preparation
  • Single staining, amplification and imaging steps
  • Manual and automated protocols

Reagents Only

  • Plug-and-play with no capital investment
  • Compatible with existing instrumentation
  • Image analysis on current software platforms
  • Single Antigen Retrieval Step

    Single Antigen Retrieval Step

    • Dewax and retrieve samples as usual
  • Single Staining Step

    Single Staining Step

    • A unique DNA sequence (barcode) is attached to each antibody
    • The staining quality of a barcoded antibody is compared to that of an unmodified antibody using serial sections to ensure staining specificity is retained
    • Barcoded antibodies are stained in a single step
  • Single Amplification Step

    Single Amplification Step

    • The ratio of barcodes per antibody is increased to bind to more fluorescent probes in the next step
    • To maximize performance, amplification is carried out after staining
    • This sensitive method detects low to highly expressed protein markers
    • All targets are amplified simultaneously
  • Single Imaging Step

    Single Imaging Step

    • DNA probes are designed to hybridize to their complementary DNA barcodes without any cross-reactivity with other barcodes
    • Probes are attached to a distinct fluorophore specific to each barcode
    • The resulting fluorescent probes bind to the amplified barcodes to generate signal for imaging in a single acquisition step
  • Whole-slide Imaging

    Whole-slide Imaging

    • InSituPlex allows for the imaging of the whole slide with no limitation to regions of interest
    • Navigate throughout the whole slide and select any area for higher resolution
  • Signal Removal

    Signal Removal

    • Fluorescent probes are gently removed while keeping barcodes intact
    • Assay starts and finishes with no fluorescence allowing for sample reuse and background signal subtraction
    • Signal removal enables higher levels of multiplexing on the same slide

Benefits

  • Spatial and quantitative marker studies for holistic data interpretation on single slides
  • Whole slide imaging with high level of multiplexing not limited to regions of interest
  • Broad, attractive, growing portfolio of kits supporting tumor immuno-profiling
  • Agile and iterative analysis of markers 
  • Robust antibody selection process with flexibility to include proprietary antibodies
  • Fully optimized reagents and protocols exempt of any assay development or optimization work
  • Plug-in assays into existing workflows with no capital investment
  • Unchanged tissue sample preparation process
  • Freedom to choose from manual and automated protocols depending on throughput needs
  • Ultivue on the way to provide content for regulated use such as CDx and Dx tests
  • Fast, batchable protocol with a single staining, single amplification, and single imaging step for 5-plex whole slide images
  • Intuitive, user-friendly kits with limited training needed
  • Agile Service offering to custom design panels and test customer samples with a fast turn-around time
  • On-demand remote and on-site support from experienced Customer Engagement team
  • Broad spectrum of kits for complete immuno-profiling studies
  • Full panel customization through Service offering
  • Access to higher level of multiplexing through strategic Partnerships
  • Wide range of marker studies from early-stage discovery to targeted, retrospective clinical validations
  • Customizable reagents with clear path for provision as regulated content

Explore Our Products

Immuno-profiling Product Kits

Our products are specifically designed for the fast and comprehensive exploration of biologically-relevant markers in tissue samples. The portfolio supports a broad range of use cases, combining level of multiplexing and sample throughput.

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Explore Our Services

Standard and Custom Services

With speed and flexibility at heart, our Services offers access to a menu of 5-Plex validated panels and to highly customizable panels to include all your markers of interests

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Client Testimonials

  • "I must say I am pretty happy with the first kit, and have high hope for the continuous improvement of the technology."

    Early Evaluator
  • "Everyone in our lab is delighted at how easy the system is."

    Early Evaluator
  • "Were are very excited with the technology, the upcoming kits and custom assay development offering."

    Early Evaluator