How It Works

DNA-based Technology

  • DNA barcode conjugated to primary antibodies
  • Complementary fluorophore-labeled probes attach to fluorophores
  • Specific, sensitive and reversible

Streamlined workflow

  • Same sample preparation
  • Single staining, amplification and imaging steps
  • Manual and automated protocols

Reagents Only

  • Plug-and-play with no capital investment
  • Compatible with existing instrumentation
  • Image analysis on current software platforms
  • Single Antigen Retrieval Step

    Single Antigen Retrieval Step

    • Dewax and retrieve antigens as usual
  • Single Staining Step

    Single Staining Step

    • A unique DNA sequence (barcode) is attached to each antibody
    • The staining quality of a barcoded antibody is compared to that of an unmodified antibody using serial sections to ensure staining specificity is retained
    • Barcoded antibodies are stained in a single step
  • Single Amplification Step

    Single Amplification Step

    • The ratio of barcodes per antibody is increased to bind to more fluorescent probes in the next step
    • To maximize performance, amplification is carried out after staining
    • This sensitive method detects low to highly expressed protein markers
    • All targets are amplified simultaneously
  • Single Imaging Step

    Single Imaging Step

    • DNA probes are designed to hybridize to their complementary DNA barcodes without any cross-reactivity with other barcodes
    • Probes are attached to a distinct fluorophore specific to each barcode
    • The resulting fluorescent probes bind to the amplified barcodes to generate signal for imaging in a single acquisition step
  • Whole-slide Imaging

    Whole-slide Imaging

    • InSituPlex allows for the imaging of the whole slide with no limitation to regions of interest
    • Navigate throughout the whole slide and select any area for higher resolution
  • Signal Removal

    Signal Removal

    • Fluorescent probes are gently removed while keeping barcodes intact
    • Assay starts and finishes with no fluorescence allowing for sample reuse and background signal subtraction
    • Signal removal enables higher levels of multiplexing on the same slide


  • Spatial and quantitative marker studies for holistic data interpretation on single slides
  • Whole slide imaging with high level of multiplexing not limited to regions of interest
  • Broad, attractive, growing portfolio of kits supporting tumor immuno-profiling for research
  • Agile analysis of marker signatures within short and iterative cycles
  • Robust antibody selection process with flexibility to include proprietary antibodies
  • Fully optimized reagents and protocols exempt of any assay development or optimization work
  • Plug-in assays into existing workflows with no capital investment
  • Unchanged tissue sample preparation process
  • Freedom to choose from manual and automated protocols depending on throughput needs
  • Ultivue on the way to provide content for regulated use such as CDx and Dx tests
  • Fast, batchable protocol with a single staining, single amplification, and single imaging step for 5-plex whole slide images
  • Intuitive, user-friendly kits with concise training needed
  • Agile Service offering to custom design panels and test customer samples with a fast turn-around time
  • On-demand remote and on-site support from experienced Customer Engagement team
  • Broad spectrum of kits for complete immuno-profiling studies
  • Full panel customization through Service offering
  • Access to higher level of multiplexing through strategic Partnerships
  • Wide range of marker studies from early-stage discovery to targeted, retrospective clinical validations
  • Customizable reagents with clear path for provision as regulated content

Explore our Technology


Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

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Explore our Services

Standard and Custom Services

With speed and flexibility at heart, our Services offers access to a menu of 5-Plex validated panels and to highly customizable panels to include all your markers of interests

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Client Testimonials

  • "I must say I am pretty happy with the first kit, and have high hope for the continuous improvement of the technology."

    Early Evaluator

  • "Everyone in our lab is delighted at how easy the system is."

    Early Evaluator

  • "Were are very excited with the technology, the upcoming kits and custom assay development offering."

    Early Evaluator