UltiMapper I/O T-act

Determine whether T cells have the potential to mediate cell death. This T cell activation 4-plex/5-color kit enables co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death. This kit contains all reagents needed to stain and image CD3, Granzyme B, Ki67, panCK / SOX10 along with a nuclear counterstain.

For Research Use Only. Not for use in diagnostic procedures.


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Marker Main Cell Type Function
CD3 T cell Identifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.
Granzyme B Cytotoxic T cells & NK cells Granzyme B is produced, stored, and released by cytotoxic cells to mediate cellular death in combination with Perforin.
Ki67 Proliferating cells Ki67 is expressed within the nucleus during the phases of mitosis and is strongly associated with cell proliferation.
CK/Sox10 Tumor cell The kit may be used to interrogate many tumor sample types for Research. A cocktail of optimized reagents for the detection of pan-Cytokeratin and Sox10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.
  • The antibody panel content of Ultivue’s kits is defined from voice-of-customer inputs, from collaborators, prospective customers and key opinion leaders.
  • Ultivue's product development process begins with the screening and careful selection of antibody clones for each target. Clones are selected for their target specificity, reliability, and widespread acceptance in clinical labs.
  • Once selected antibodies have been conjugated to unique DNA barcodes, they are tested to verify that the antibody conjugates retain the same performance as the unconjugated material. This is carried out by comparing the staining quality with gold standard methods and certifying the staining by a qualified reviewer.
  • To establish multiplexed panels, a titration series for each antibody conjugate is carried out in FFPE tissue control samples to select optimal antibody staining concentrations. Assay protocol steps are optimized as needed.
  • When applicable, the analytical sensitivity of the assay for a given target is tested using standards with a known concentration gradient, such as cell pellet controls.
  • To confirm reproducibility specifications, functional testing of the entire kit is accomplished via inter-instrument, inter-day, inter-analyst, and intra-run testing for all protocols. Samples are imaged on a wide range of microscopes and slide scanners from leading suppliers.
  • Images from reference samples are formally reviewed by consulting pathologists. Alpha and/or Beta pre-production kits are evaluated by prospective customer labs to ensure the assay meets performance expectations and that the kit documents and instructions are clear and complete.
  • Multiple stability tests are run for every component of the kit including real-time, accelerated, in-use, on-board, shipping, and freeze-thaw cycle stability.
  • The kits for immuno-profiling are designed to offer seamless integration into standard immunohistochemistry research workflows. The optimized manual and automated protocols enable a rapid path from sample to answer in a single day.
  • The kit is configured to offer an easy-to-use, fast, and fully automated tissue staining solution using the Leica BOND RX® system. The reagents enable the detection of up to four markers and a nuclear counterstain on whole slides using research fluorescence tissue scanners such as the Leica Aperio VERSA® amongst other validated imaging solutions. The resulting multiplexed images are compatible with most digital pathology image analysis solutions, such as the IndicaLabs Halo® platform, enabling the automated quantification of spatial and cellular expression data over whole tissue sections.
  • As an example, our kits enable the automated staining of four targets and nuclear counterstain on up to 30 slides at one time on the Leica BOND RX®, in 5 hours. Using current imaging systems, a 2 cm2 FFPE section can be imaged in five fluorescent channels in less than 15 minutes, enabling a true sample to answer workflow within a work day.