Published June 25, 2018

Multiplexing in Whole-Slide Tissue Imaging for Life Science

Researchers and clinicians must work across biomarker discovery and companion diagnostic areas to translate the biological complexity of a disease into actionable information. High-content data generation and analysis – covering both the detection of what biomarkers are present and an understanding of how they interact in a particular microenvironment – is required to enable this effort.

With high spatial heterogeneity and biological complexity, resected tumor samples present a characterization challenge, particularly when samples are scarce. Multiplexing in a single, intact tissue sample is required to identify cell types, understand biological pathways, and map cellular interactions. Ultivue has developed a platform reagent technology (InSituPlexTM) that simultaneously enables whole-slide higher multiplexing by immunofluorescence and complete compatibility with the ZEISS Axio Scan.Z1 slide scanning system.

Advantages

  • High Multiplexing In Situ Detect 5 or more markers in a single sample with whole slide imaging
  • Sample Fully Preserved Complete retention of sample antigenicity through the assay for unbiased biology
  • Plug-in Validated on multiple tissue types using ZEISS Axio Scan.Z1
  • Improved workflow Single staining step and simultaneous signal amplification for all markers lowers time to answer
  • Versatile implementation Same technology, same reagents across the Translational Medicine value chain: equally applicable from Discovery to downstream high throughput testing
  • Ultivue has developed a platform reagent technology (InSituPlexTM) that simultaneously enables whole-slide higher multiplexing by immunofluorescence and complete compatibility with the ZEISS Axio Scan.Z1 slide scanning system.

Performance

  • Whole Slide Imaging Axio Scan.Z1 imaging of up to 100 slides with fast and stable LEDs and sensitive high-speed sCMOS cameras.
  • Exceptional Image Quality Highest resolution 10x, 20x and 40x objectives provide excellent images for easier downstream analysis.
  • One system for brightfield, fluorescence and polarization microscopy
  • Modular tray concept allows to use a large range of slides
  • Flexible system for demanding applications
  • Easy to operate

Download the Application Note Here

About Ultivue

Ultivue develops innovative Research Use Only reagent-based solutions that maximize the biological information available from cells and tissue samples. The company's patent-pending technology is being used to elucidate biological pathways and to validate clinically-relevant biomarkers. Ultivue is based in Cambridge, MA. To learn more, visit www.ultivue.com

Contact

Louis Levy
617-945-2662
contact@ultivue.com